lentiviral transfer vector (Addgene inc)
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Addgene inc
lentiviral transfer vector
Lentiviral Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+transfer+vector/psPAX2+(Plasmid+%2312260)/pm41597202-77-4-10
Average 98 stars, based on 15580 article reviews
Lentiviral Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 15580 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+transfer+vector/psPAX2+(Plasmid+%2312260)/pm41597202-77-4-10
Average 98 stars, based on 15580 article reviews
lentiviral transfer vector - by Bioz Stars,
2026-10
98/100 stars
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Knockdown:Article Title: Inhibitory co-receptor Lag3 supports Foxp3 + regulatory T cell function by restraining Myc-dependent metabolic programming. Article Snippet: Article CRISPR:Article Title: Inhibitory co-receptor Lag3 supports Foxp3 + regulatory T cell function by restraining Myc-dependent metabolic programming. Article Snippet: Article Clone Assay:Article Title: Inhibitory co-receptor Lag3 supports Foxp3 + regulatory T cell function by restraining Myc-dependent metabolic programming. Article Snippet: Article Transfection:Article Title: Targeting of Human Mitochondrial DNA with Programmable pAgo Nuclease. Article Snippet: HEK293T cells were seeded in 6-well plates at a density of 1 × 106 cells per well to reach approximately 80% confluency after 24 h. Two hours prior to transfection, the culture medium was replaced with serum-free Opti-MEM (Thermo Fisher Scientific). .. For lentiviral transfection, the Article Title: Targeting of Human Mitochondrial DNA with Programmable pAgo Nuclease Article Snippet: HEK293T cells were seeded in 6-well plates at a density of 1 × 10 6 cells per well to reach approximately 80% confluency after 24 h. Two hours prior to transfection, the culture medium was replaced with serum-free Opti-MEM (Thermo Fisher Scientific). .. For lentiviral transfection, the Plasmid Preparation:Article Title: Targeting of Human Mitochondrial DNA with Programmable pAgo Nuclease. Article Snippet: HEK293T cells were seeded in 6-well plates at a density of 1 × 106 cells per well to reach approximately 80% confluency after 24 h. Two hours prior to transfection, the culture medium was replaced with serum-free Opti-MEM (Thermo Fisher Scientific). .. For lentiviral transfection, the Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells Article Snippet: To knockdown the expression of KRAS, CRISPR/dCas9 system was adopted to target the promoter site of murine Kras gene. sgRNA target sequences were designed using CRISPick ( https://portals.broadinstitute.org/gppx/crispick/public ) with reference genome as “mouse GRCm38 (NCBI RefSeq v.108)” and enzyme as “SpyoCas9 (NGG)”. .. Candidate oligomers for sgRNA were generated by adding respectively the overhang sequences “CACC” and “AAAC” to the 5′ end of forward and reverse oligos to facilitate the cloning of oligomers into the BsmBI sites in the Article Title: Functional dissection of metabolic trait-associated gene regulation in steady state and stimulated human skeletal muscle cells Article Snippet: We sequenced the resulting libraries on an Illumina NovaSeq 6000 and received paired-end 150-bp reads. .. To deliver the MPRA library to the LHCN-M2 human skeletal myoblasts, we ported the assembled MPRA block (oligo, barcode, promoter, GFP) to a Article Title: Targeting of Human Mitochondrial DNA with Programmable pAgo Nuclease Article Snippet: HEK293T cells were seeded in 6-well plates at a density of 1 × 10 6 cells per well to reach approximately 80% confluency after 24 h. Two hours prior to transfection, the culture medium was replaced with serum-free Opti-MEM (Thermo Fisher Scientific). .. For lentiviral transfection, the Article Title: Using a modular massively parallel reporter assay to discover context-specific regulatory grammars in type 2 diabetes Article Snippet: .. For use with the LHCN-M2 skeletal muscle myocyte cell line, we ported the assembled MPRA block (fragment, barcode, promoter, GFP) to a Generated:Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells Article Snippet: To knockdown the expression of KRAS, CRISPR/dCas9 system was adopted to target the promoter site of murine Kras gene. sgRNA target sequences were designed using CRISPick ( https://portals.broadinstitute.org/gppx/crispick/public ) with reference genome as “mouse GRCm38 (NCBI RefSeq v.108)” and enzyme as “SpyoCas9 (NGG)”. .. Candidate oligomers for sgRNA were generated by adding respectively the overhang sequences “CACC” and “AAAC” to the 5′ end of forward and reverse oligos to facilitate the cloning of oligomers into the BsmBI sites in the Cloning:Article Title: Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cells Article Snippet: To knockdown the expression of KRAS, CRISPR/dCas9 system was adopted to target the promoter site of murine Kras gene. sgRNA target sequences were designed using CRISPick ( https://portals.broadinstitute.org/gppx/crispick/public ) with reference genome as “mouse GRCm38 (NCBI RefSeq v.108)” and enzyme as “SpyoCas9 (NGG)”. .. Candidate oligomers for sgRNA were generated by adding respectively the overhang sequences “CACC” and “AAAC” to the 5′ end of forward and reverse oligos to facilitate the cloning of oligomers into the BsmBI sites in the Article Title: Overexpression of NKG2D and IL24 in NK Cell-Derived Exosomes for Cancer Therapy Article Snippet: The human NKG2D gene (GenBank: NM_007360) and IL24 gene (GenBank: NM_006850) were amplified by PCR using high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Restriction sites suitable for insertion into the multiple cloning site (MCS) of the Article Title: Functional dissection of metabolic trait-associated gene regulation in steady state and stimulated human skeletal muscle cells Article Snippet: We sequenced the resulting libraries on an Illumina NovaSeq 6000 and received paired-end 150-bp reads. .. To deliver the MPRA library to the LHCN-M2 human skeletal myoblasts, we ported the assembled MPRA block (oligo, barcode, promoter, GFP) to a Article Title: Overexpression of NKG2D and IL24 in NK Cell-Derived Exosomes for Cancer Therapy Article Snippet: The human NKG2D gene (GenBank: NM_007360) and IL24 gene (GenBank: NM_006850) were amplified by PCR using high-fidelity DNA polymerase (Thermo Fisher Scientific, Waltham, MA, USA). .. Restriction sites suitable for insertion into the multiple cloning site (MCS) of the Article Title: Using a modular massively parallel reporter assay to discover context-specific regulatory grammars in type 2 diabetes Article Snippet: .. For use with the LHCN-M2 skeletal muscle myocyte cell line, we ported the assembled MPRA block (fragment, barcode, promoter, GFP) to a Blocking Assay:Article Title: Functional dissection of metabolic trait-associated gene regulation in steady state and stimulated human skeletal muscle cells Article Snippet: We sequenced the resulting libraries on an Illumina NovaSeq 6000 and received paired-end 150-bp reads. .. To deliver the MPRA library to the LHCN-M2 human skeletal myoblasts, we ported the assembled MPRA block (oligo, barcode, promoter, GFP) to a Article Title: Using a modular massively parallel reporter assay to discover context-specific regulatory grammars in type 2 diabetes Article Snippet: .. For use with the LHCN-M2 skeletal muscle myocyte cell line, we ported the assembled MPRA block (fragment, barcode, promoter, GFP) to a |